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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been observed in many tumors, which results in chromosome instability, and so may possibly add to tumorigenesis. This protein was observed to associate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital function in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but detailed nucleotide sequence facts just isn't nonetheless offered.

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Blocking peptides are peptides that bind specially towards the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.

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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...

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This tutorial outlines further more probable optimization of mobile and immunoassay parameters to ensure the best possible results are...

Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By evaluating the staining within the blocked antibody vs . the antibody by yourself, one can see which staining is certain.

Goal Info Cyclin E belongs to the highly conserved cyclin spouse and children, whose users are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.

The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...

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Clicking the images or hyperlinks will redirect you to an internet site hosted by BenchSci that gives 3rd-bash scientific content. Neither the content material nor the BenchSci technology and processes for selection are already evaluated by us; we've been providing them as-is and with out warranty of any type, such as for use or application of the Thermo Fisher Scientific goods presented.